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Systematic Review Assisted Reproduction

Air quality control on in vitro fertilization outcomes in assisted reproduction laboratories: a systematic review

Bruna Sabbag Pontes, Marília Leão Pereira Resende, Eduardo Camelo de Castro

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Objectives: Embryology laboratories’ air quality affects the success of human assisted reproduction. In this paper we made a review of studies that assessed the importance of air quality control of embryology laboratories in the success of assisted human reproduction. Methods: A systematic review was made with studies presented in the following database: PUBMED, Lilacs and Scielo submitted. The researched articles refer to the years 1996 to 2016, in Portuguese, English and Spanish. The keywords were air quality control, in vitro fertilization, embryology, assisted human reproduction, laboratory environment, volatile organic compounds and clean room. Results: Thirty three articles were found. From these, eight studies fulfilled the inclusion criteria. All of them used air filters. Conclusions: The reviewed studies show a trend of improvement in pregnancy rates due to the introduction of filters in IVF laboratories. Apparently, the greater the level of control of environmental pollution and elimination of VOCs.


Air quality; in vitro fertilization; HEPA filter; VOC filter.


1. Cohen J, Gilligan A, Esposito W, Schimmel T, Dale B. Ambient air and its potential effects on conception in vitro. Hum Reprod. 1997;12(8):1742-9. PMid:9308805.
<br/><br/>2. Bento FC and Esteves S. Implementation of cleanroom technology in reproductive laboratories: the question is not why but how. Reprod Biomed Online. 2016;32(1):9-00311. PMid:26602944.
<br/><br/>3. Esteves SC, Bento FC. Air quality control in the ART laboratory is a major determinant of IVF success. Asian J Androl. 2016;18(4):596- 599. PMid:26585700.
<br/><br/>4. Esteves SC, Bento FC. Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive. Reprod Biomed Online. 2013;26(1):9-21. PMid:23177417.
<br/><br/>5. Boone WR, Johnson JE, Locke AJ, Crane MM 4th, Price TM. Control of air quality in an assisted reproductive technology laboratory. Fertil Steril. 1999;71(1):150-4. PMid:9935133.
<br/><br/>6. Gomes AP Junior SV, Esteves S. O controle da poluição do ar no laboratório de fertilização in vitro e áreas adjacentes melhora as taxas de formação de embriões, clivagem e de gravidez e diminui a taxa de aborto: comparação entre salas limpas classe 100 (ISO 5) e classe 1.000 (ISSO 6). 2003.
<br/><br/>7. Jindal SK, Polotsky AJ, Buyuk ER, Lieman HJ, Gilligan A. Improved pregnancy rates following introduction of engineering controls of lab air quality. Fertil Steril. 2008;90(Suppl):S403.
<br/><br/>8. Dickey RP, Wortham JWE Jr, Potts A, Welch A. Effect of IVF laboratory air quality on pregnancy success. Fertil Steril. 2010;94(4):S151.
<br/><br/>9. Khoudja RY, Xu Y, Li T, Zhou C. Better IVF outcomes following improvements in laboratory air quality. J Assist Reprod Genet. 2013;30(1):69-76. PMid:23242648.
<br/><br/>10. Forman M, Sparks AET, Degelos S, Koulianos G, Worrilow KC. 10 Statistically significant improvements in clinical outcomes using engineered molecular media and genomically modeled ultraviolet light for comprehensive control of ambient air (AA) quality. Fertil Steril. 2014;102(3):e91.
<br/><br/>11. Munch EM, Sparks AE, Duran HE, Van Voorhis BJ. Lack of carbon air filtration impacts early embryo development. J Assist Reprod Genet. 2015;32(7):1009-17. PMid:26003657.
<br/><br/>12. Morbeck DE. Air quality in the assisted reproduction laboratory: a mini-review. J Assist Reprod Genet. 2015;32(7):1019-24. PMid:26238385.

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