Human Reproduction Archives
http://humanreproductionarchives.com/article/doi/10.4322/hra.000717
Human Reproduction Archives
Research Article Assisted Reproduction

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Tamires Correia Evangelista Dutra, Daniela Scherer da Silva, Virgínia Meneghini Lazzari2, Alberto Stein, João Sabino da Cunha Filho

Downloads: 0
Views: 437

Abstract

Introduction: 50% of pregnancy failure is due male factors. One of these factors is the absence of spermatozoa in the ejaculate; in these cases the patient is submitted to epididymal or testicular sperm aspiration. The spermatozoa acquired from these technics usually are immobile, what makes necessary the use of pentoxifylline, which activates sperm movement, to distinction of viable spermatozoa to ICSI. Objectives: To analyze if a low concentration of pentoxifylline causes spermatic chromatin damages. Materials and Methods: Sperm motility activation was tested in different concentrations of pentoxifylline. After the screening of doses, the lowest effective concentration was choose to analyze the chromatin damage rate. 15 samples of fresh sperm were tested for DNA damage with HALOSPERM Kit. Blades’ analyses were made in bright field microscopy. The fragmented DNA was settle by the absence of chromatin dispersion halo or small halo. Results: The lowest and effective pentoxifylline dose was 1.5 mM. There was no significant difference on chromatin damage rate between study and control groups (p = 0.55). Conclusion: The use of pentoxifylline at 1.5 mM does not affect the sperm DNA.

Keywords

pentoxifylline; sperm motility; DNA fragmentation.

References

1. Inhorn MC. Global infertility and the globalization of new reproductive technologies: illustrations from Egypt. Soc Sci Med. 2003;56(9):1837-51. PMid:12650724. http://dx.doi.org/10.1016/S0277-9536(02)00208-3.
<br/><br/>2. Brasil. Ministério da Saúde. Portaria nº 426/GM em 22 de março de 2005. Diário oficial [da] República Federativa do Brasil [Internet]; Brasilia [cited 2016 May 16]. Available from: http://bvsms.saude.gov.br/bvs/publicacoes/portaria_426_ac.htm
<br/><br/>3. Izzo CR, Monteleone PAA, Serafini PC. Human reproduction: current status. Rev Assoc Med Bras. 2015;61(6):557-9. PMid:26841167. http://dx.doi.org/10.1590/1806-9282.61.06.557.
<br/><br/>4. Pasqualotto FF. Investigação e reprodução assistida no tratamento da infertilidade masculina. Rev Bras Ginecol Obstet. 2007;29(2):103-12. http://dx.doi.org/10.1590/S0100-72032007000200008.
<br/><br/>5. Rosenblatt C, Delgado Fo MA, Delgado DR, Delgado FR. Infertilidade masculina: novos conceitos. Prat. Hosp. 2010;7:85-92.
<br/><br/>6. Esteves SC, Miyaoka R, Orosz JE, Agarwal A. An update on sperm retrieval techniques for azoospermic males. Clinics. 2013;68(Suppl 1):99. PMid:23503959. http://dx.doi.org/10.6061/clinics/2013(Sup01)11.
<br/><br/>7. Dzik A, Donadio NF, Esteves SC, Nagy ZP. Atlas de reprodução humana. São Paulo: SBRH; 2010. p. 14-15.
<br/><br/>8. Oberst ER, Tarouco LMR, Konrath MLP, Vidor S. Célula espermática: projeto e desenvolvimento de objetos educacionais [Internet]. Porto Alegre: UFRGS; 2016 [cited 2016 May 2]. Available from: http://penta3.ufrgs.br/veterinaria/celula/
<br/><br/>9. Manetta LA. Efeito da pentoxifilina sobre a motilidade e concentração in vitro dos espermatozoides humanos em indivíduos inférteis com oligoastenozoospermia e astenozoospermia. Ver. Bras. Ginec. Obst. 1998;20(1):53. http://dx.doi.org/10.1590/S0100-72031998000100011.
<br/><br/>10. Unsal E, Turan V, Aktuna S, Hurdag C, Bereketoglu G, Canillioglu Y, et al. Effects of pentoxifylline and platelet activating factor on sperm DNA damage. Eur J Obstet Gynecol Reprod Biol. 2016;197:125-9. PMid:26748389. http://dx.doi.org/10.1016/j.ejogrb.2015.12.016.
<br/><br/>11. Souza AP, Santos JR, Santos TA. A importância da integridade da cromatina dos espermatozoides na infertilidade masculina. Acta Urol. Port.Coimbra. 2010;27(2):7-47.
<br/><br/>12. Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21(1):33-44. PMid:10670517.
<br/><br/>13. Henkel R, Kierspel E, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, et al. DNA fragmentation of spermatozoa and assited reproduction technology. RBM Online. 2003;4(7):477-84. PMid:14656411.
<br/><br/>14. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assited reproductive technique. Hum Reprod. 2003;18(5):1023-8. PMid:12721180. http://dx.doi.org/10.1093/humrep/deg228.
<br/><br/>15. Piasecka M, Gaczarzewicz D, Laszczynska M. Evaluation of sperm genomic integrity of normozoospermic men: a prospective study. Folia Histochem Cytobiol. 2006;44(2):117-22. PMid:16805137.

5a7c36950e8825cc2cb51848 hra Articles
Links & Downloads

Hum Reprod Arch

Share this page
Page Sections